Phân tử chẩn đoán của một bệnh nhân nhỏ mắt Toxocariasis tại Việt Nam

Tháng Bảy 21, 2017

Nguyen Van De1,*, Nguyen Vu Trung2, Le Van Duyet2 and Jong-Yil Chai3
1Department of Parasitology, Hanoi Medical University, Hanoi, Vietnam; 2National Hospital of Tropical Diseases, Hanoi, Vietnam; 3Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea
Abstract: An ocular Toxocara canis infection is reported for the first time in Vietnam. A 34-year-old man residing in a village of Son La Province, North Vietnam, visited the National Eye Hospital (NEH) in August 2011. He felt a bulge-sticking pain in his left eye and loss of vision occurred over 3 months before visiting the hospital. The eye examination in the hospital showed damage of the left eye, red eye, retinal fibrosis, retinal detachment, inflammation of the eye tissues, retinal granulomas, and a parasitic cyst inside. A larva of Toxocarawas collected with the cyst by a medical doctor by surgery. Comparison of 264 nucleotides of internal transcribed spacer 2 (ITS2) of ribosomal DNA was done between our Vietnamese Toxocara canis and other Toxocara geographical isolates, including Chinese T. canis, Japanese T. canis, Sri Lankan T. canis, and Iranian T. canis. The nucleotide homology was 97-99%, when our T. canis was compared with geographical isolates. Identification of a T. canis infection in the eye by a molecular method was performed for the first time in Vietnam.
Key words: Toxocara canis, case report, eye, molecular diagnosis, PCR, ITS2, Vietnam

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