Chuyên mục: Nghiên cứu khoa học

Tháng Chín 11, 2017

Case report: unusual presentation of Fasciolopsis
buski in a Viet Namese child
Thanh Hoa Le a, Van De Nguyen b, Ba Uoc Phan c, David Blair d,
Donald P. McManus e,*
a>Institute of Biotechnology (IBT), Hanoi, Viet Nam
b>National Institute for Malariology, Parasitology and Entomology (NIMPE), Hanoi, Viet Nam
c>Centre for Control of Malaria and Parasites, Nghe an Province, Viet Nam
d>School of Tropical Biology, James Cook University, Townsville, Queensland, Australia
e> Molecular Parasitology Laboratory, Australian Centre for International and Tropical Health and
Nutrition, Queensland Institute of Medical Research and University of Queensland, 300 Herston Road,
Q4029, Brisbane, Queensland, Australia
Received 9 May 2003; received in revised form 23 June 2003; accepted 24 June 2003
” Summary A Viet Namese child presented with a history of abdominal pain. Shortly
afterwards, he vomited eight live trematode flukes that were collected and morphologically identified as Fasciolopsis buski. The identification was confirmed by DNA
analysis. Adult worms of F. buski from humans are very rarely seen except at autopsy,
and this is the first such report from Viet Nam”
© 2003 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All
rights reserved”

Tháng Chín 5, 2017

Molecular Diagnosis of an Ocular Toxocariasis Patient in Vietnam
Korean J Parasitol Vol, 51, No.5:1-5, October 2013
Nguyen Van De, Nguyen Vu Trung, Le Van Duyet and Jong-Yil Chai
Department of Parasitology, Hanoi Medical University, Hanoi, Vietnam: National Hospital of Tropical Diseases, Hanoi, Vietnam, Department of Parasitology and Tropical Medicine, Seoul National College of Medicine, Seoul 110-799, Korea
Abstract: An ocular Toxocara canis infection is reported for the first time in Vietnam. A 34-year- old man residing in a vil-lage of Son La Province, North Vitnam, visited the National Eye Hospital ( NEH) in August 2011. He felt a bulge-sticking pain in his left eye and loss of vision occurred over 3 months before visiting the hospital. The eye examination in the hospital showed damage of the left eye , red eye, retinal fibrosis, retinal detachment, inflammation of the eye tissues, retinal granulomas, and a parasitic cyst inside. A larva of Toxxocara was collected with the cyst by a medical doctor by surgery. Comparison of 264 nucleotides of internal transcribed spacer 2 (ITS2) of ribosomal DNA was done between our Vietnamese Toxocara canis and other Toxxocara geographical isolates, including Chinese T.canis, Japanese T. canis, Sri LankaT.canis, and Iranian T. Canis. The nucleotide homology was 97-99%, when our T.canis was compared with geographical isolates, Identification of a T.canis infection in the eye by a molecular method was performed for the first time in Vietnam.
Key words: Toxocara canis, case report, eye, molecular, PCR, ITS2, Vietnam

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Tháng Bảy 21, 2017

Prevalence of Soil-Transmitted Helminths and Molecular Clarification of Hookworm Species in Ethnic Ede Primary Schoolchildren in Dak Lak Province, Southern Vietnam Bui Khac Hung1, Nguyen Van De2,*, Le Van Duyet3, Jong-Yil Chai4,5 1Krong Pac Hospital, Dak Lak Province, Vietnam, 2Hanoi Medical University, Hanoi, Vietnam, 3National Hospital of Tropical Diseases (NHTD), Hanoi, Vietnam; 4Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 03080, Korea; 5Korea Association of Health Promotion, Seoul 03722, Korea Abstract: To know the infection status of helminths in primary schoolchildren of southern parts of Vietnam, we performed an epidemiological study in Krong Pac district, Dak Lak Province, Vietnam. A total of 1,206 stool specimens were collected from ethnic Ede schoolchildren in 4 primary schools in 2015 and examined by the Kato-Katz technique. In addition, stool cultures were done by the Harada-Mori method to obtain hookworm larvae and then to clarify the species of hookworms infected. The results showed that the helminth infection rate was 25.0%, including 2.0% Ascaris lumbricoides, 0.33% Trichuris trichiura, and 22.8% hookworm infections. The average intensity of infection was 102.0 eggs per gram of feces (EP

Tháng Bảy 21, 2017

PREVALENCE AND SPECIES OF Fasciolopsis buski IN VIETNAM Nguyen Van De and Pham Ngoc Minh Hanoi Medical University, Hanoi Vietnam Abstract PERPOSE: To report prevalence and species of Fasciolopsis buski in Vietnam. METHOD: Stool examination by Kato-Katz technique for detection prevalence of fasciolopsiasis, and identification species by morphology and molecular method. RESULT: On 10,540 stool samples were examined in 14 provinces and adult worms of Fasciolopsis collected from patients in other 3 provinces. The result showed that 13/14 provinces have been patients infected Fasciolopsis. However, fasciolopsiasis was determined distribute in 16 provinces with prevalence of 1.23% in average (from 0.16% to 3.82%). Adult worms were identified as Fasciolopsis buski by morphology and confirmed by molecular analysis using of 1950 bp-18S ribosomal RNA(18S rRNA) as genetic marker. Keywords: fasciolopsiasis, Fasciolopsis buski, Vienam. http://www.mediafire.com/file/7130oghq7qhsvd0/Fasciolopsis_buski_IN_VIETNAM.pdf

Tháng Bảy 21, 2017

Nguyen Van De1,*, Nguyen Vu Trung2, Le Van Duyet2 and Jong-Yil Chai3 1Department of Parasitology, Hanoi Medical University, Hanoi, Vietnam; 2National Hospital of Tropical Diseases, Hanoi, Vietnam; 3Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea Abstract: An ocular Toxocara canis infection is reported for the first time in Vietnam. A 34-year-old man residing in a village of Son La Province, North Vietnam, visited the National Eye Hospital (NEH) in August 2011. He felt a bulge-sticking pain in his left eye and loss of vision occurred over 3 months before visiting the hospital. The eye examination in the hospital showed damage of the left eye, red eye, retinal fibrosis, retinal detachment, inflammation of the eye tissues, retinal granulomas, and a parasitic cyst inside. A larva of Toxocarawas collected with the cyst by a medical doctor by surgery. Comparison of 264 nucleotides of internal transcribed spacer 2 (ITS2) of ribosomal DNA was done between our Vietnamese Toxocara canis and other Toxocara geographical isolates, including Chinese T. canis, Japanese T. canis, Sri Lankan T. canis, and Iranian T. canis. The nucleotide homology was 97-99%, when our T. canis was compared with geographical isolates. Identification of a T. canis infection in the ey

Tháng Bảy 21, 2017

Nguyễn Văn Đề, Phạm Ngọc Minh Bộ môn Ký sinh trùng, Trường Đại học Y Hà Nội Tóm tắt Ấu trùng sán dây chó thu thập từ 2 bệnh nhân (bệnh nhân nam 42 tuổi ở Thanh Hóa và bệnh nhân nữ 48 tuổi ở Sơn La) năm 2013 đã được xác định loài. Các ấu trùng có hình cầu kích thước 0,15 x 0,14mm với 4 hấp khẩu và 30 móc, mỗi móc có chiều dài 22µm. Bằng phương pháp hình thái học và sinh học phân tử sử dụng gen NADH dehyrogenase 1 với kỹ thuật PCR, các ấu trùng này được xác định là Echinococcus ortleppi (với mức độ tương đồng nucleotide với các chủng chuẩn trong Ngân hàng gen là 99-100%). Đây là loài lần đầu tiên được phát hiện trên người tại Việt Nam. Từ khóa: Phổi, Echinococcus ortleppi, hydatid cyst disease, Việt Nam Toàn văn dowload tại đây: http://www.mediafire.com/file/46zug85y191i12f/Echinococcus+VN-PCR.pdf

Tháng Bảy 21, 2017

Abstract

Several reports on taeniasis and cysticercosis in Vietnam show that they are distributed in over 50 of 63 provinces. In some endemic areas, the prevalence of taeniasis was 0.2-12.0% and that of cysticercosis was 1.0-7.2%. The major symptoms of taeniasis included fidgeted anus, proglottids moving out of the anus, and proglottids in the feces. Clinical manifestations of cysticercosis in humans included subcutaneous nodules, epileptic seizures, severe headach, impaired vision, and memory loss. The species identification of Taenia in Vietnam included Taenia asiatica, Taenia saginata, and Taenia solium based on combined morphology and molecular methods. Only T. solium caused cysticercosis in humans. Praziquantel was chosen for treatment of taeniasis and albendazole for treatment of cysticercosis. The infection rate of cysticercus cellulosae in pigs was 0.04% at Hanoi slaughterhouses, 0.03-0.31% at provincial slaughterhouses in the north, and 0.9% in provincial slaughterhouses in the southern region of Vietnam. The infection rate of cysticercus bovis in cattle was 0.03-2.17% at Hanoi slaughterhouses. Risk factors investigated with regard to transmission of Taenia suggested that consumption of raw meat (eating raw meat 4.5-74.

Tháng Bảy 21, 2017

Paiboon Sithithaworn a,b,⁎, Ross H. Andrews a,b, Nguyen Van De c, Thitima Wongsaroj d, Muth Sinuon e, Peter Odermatt f, Yukifumi Nawa g, Song Liang h, Paul J. Brindley i, Banchob Sripa b, j,⁎⁎ a Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand b Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand c Department of Parasitology, Hanoi Medical University, Hanoi, Vietnam d Department of Disease Control, Ministry of Public Health, Thailand e Centre for Parasitology, Entomology and Malaria Control, Phnom Penh, Cambodia f Swiss Tropical and Public Health Institute, Basel, Switzerland g Research Affairs, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand h Ohio State University, Columbus, OH, USA i Department of Microbiology, Immunology & Tropical Medicine, School of Medicine & Health Sciences, The George Washington University, Washington, DC 20037, USA j Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand a r t i c l e i n f o a b s t r a c t Article history: Received 4 August 2011 Accepted 9 August 2011 Available online 25 August 2011 Keywords: Opisthorchis viverrini Clonorchis sinensis Prevalence Lower Mekong Basin Current statatus ABSTRACT This review highlights the current status and control of liver

Tháng Bảy 21, 2017

Abstract Trichinellosis is an important emerging or re-emerging zoonotic disease in Southeast Asia. In Vietnam, data on Trichinellosis are scarce. Therefore, the present study was designed to determine the seroprevalence of Trichinellosis in the domestic lifecycle in two provinces of northwestern Vietnam, where recently isolated outbreaks of human trichinellosis occurred. Serum samples were obtained from 558 pigs, 125 dogs and 98 cats, transported on filter paper, and tested for Trichinella antibodies by ELISA and Western blot, using larval excretory–secretory (E/S) antigens. The overall seroprevalence of antibodies to Trichinella was 5.6%, 4% and 0% in pigs, dogs and cats, respectively. In pigs, positive cases were distributed in 8/20 districts of the two provinces. This study suggests that Trichinella spp. is circulating in the domestic life cycle in northwestern Vietnam. Further study is recommended to investigate the presence of Trichinella in a sylvatic cycle, and to identify the occurring Trichinella species. Chi tiết download tại: http://www.mediafire.com/file/ezkkj5rw7ekqiwc/Nga-Trichinella+on+domestic+-2013.pdf

Tháng Bảy 21, 2017

Thanh Hoa Le,a Nga Thi Bich Nguyen,a Nam Hai Truong,b and Nguyen Van Dec Department of Immunology, Institute of Biotechnology, Vietnam Academy of Science and Technology (VAST), Cau Giay District, Hanoi, Vietnama; Department of Genetic Engineering, Institute of Biotechnology, VAST, Cau Giay District, Hanoi, Vietnamb; and Department of Parasitology, Hanoi Medical University, Dong Da District, Hanoi, Vietnamc Mitochondrial DNA sequences offer major advantages over the more usual nuclear targets for loop-mediated isothermal amplification approaches (mito-LAMP) because multiple copies occur in every cell. Four LAMP primers [F3, FIP(F1cF2), BIP(B1cB2), and B3] were designed based on the mitochondrial nad1 sequence of Opisthorchis viverrini and used for a highly specific assay (mito-OvLAMP) to distinguish DNA of O. viverrini from that of another opisthorchiid (Clonorchis sinensis) and other trematodes (Haplorchis pumilio, Haplorchis taichui, Fasciola hepatica, and Fasciola gigantica). Conventional PCR was applied using F3/B3 primer pairs to verify the specificity of the primers for O. viverrini DNA templates. All LAMP-positive samples could be detected with the naked eye in sunlight, by gel electrophoresis (stained with ethidium bromide), and by addition of SYBR green I to the product in sunlight or under UV light