Diroflaria repens in Vietnam: Detection of 10 Eye and Subcutaneous Tissue Infection Cases Identifed by Morphology and Molecular Methods

Tháng Mười 11, 2017

Diroflaria repens in Vietnam: Detection of 10 Eye and
Subcutaneous Tissue Infection Cases Identifed by
Morphology and Molecular Methods

Nguyen Van De1,*, Thanh Hoa Le2 and Jong-Yil Chai3
1Department of Parasitology, Hanoi Medical University, Hanoi, Vietnam; 2Department of Immunology, Institute of Bio-Technology, Hanoi, Vietnam;
3Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea

Abstract: From 2006 to 2010, hospitals in Hanoi treated 10 human patients for diroflariasis. The worms were collected
from parasitic places, and identifcation of the species was completed by morphology and molecular methods. Ten parasites were recovered either from the conjunctiva (n=9) or subcutaneous tissue (n=1). The parasites were 4.0-12.5 cm in
length and 0.5-0.6 mm in width. Morphological observations suggested all parasites as
Diroflaria repens. Three of the 10
parasites (1 from subcutaneous tissue and 2 from eyes) were used for molecular confrmation of the species identifcation. A portion of the mitochondrial
cox1 (461 bp) was amplifed and sequenced. Nucleotide and amino acid homologies
were 95% and 99-100%, respectively, when compared with
D. repens (Italian origin, GenBank AJ271614; DQ358814).
This is the frst report of eye diroflariasis and the second report of subcutaneous tissue diroflariasis due to
D. repens in
Vietnam.
Key words: Diroflaria repens, case report, eye, subcutaneous tissue, PCR, mitochondrial cox1, Vietnam

https://drive.google.com/file/d/0BwAVbkCHjoJ8TzVTZS1lWmlxems/view?usp=sharing