An Outbreak of Trichinosis with Molecular Identifcation of Trichinella sp. in Vietnam

Tháng Mười 11, 2017

An Outbreak of Trichinosis with Molecular Identifcation
of
Trichinella sp. in Vietnam

Nguyen Van De1,*, Nguyen Vu Trung2, Nguyen Hong Ha2, Vu Thi Nga3, Nguyen Minh Ha2, Pham Thanh Thuy4,
Le Van Duyet
2 and Jong-Yil Chai5
1Department of Parasitology, Hanoi Medical University, Vietnam; 2National Hospital of Tropical Diseases, Hanoi, Vietnam; 3National Centre for
Veterinary Diagnosis, Hanoi, Vietnam;
4Bach Mai Hospital, Hanoi, Vietnam; 5Department of Parasitology and Tropical Medicine, Seoul National
University College of Medicine, Seoul 110-799, Korea


Abstract: The 5th outbreak of trichinosis occurred in a mountainous area of North Vietnam in 2012, involving 24 patients
among 27 people who consumed raw pork together. Six of these patients visited several hospitals in Hanoi for treatment.
Similar clinical symptoms appeared in these patients within 5-8 days after eating infected raw pork, which consisted of
fever, muscle pain, diffcult moving, edema, diffcult swallowing, and diffcult breathing. ELISA revealed all (6/6) positive
reactions against
Trichinella spiralis antigen and all cases showed positive biopsy results for Trichinella sp. larvae in the
muscle. The larvae detected in the patients were identifed as
T. spiralis (Vietnamese strain) by the molecular analysis of
the mitochondrial cytochrome
c oxidase subunit III (cox3) gene.
Key words: Trichinella spiralis, muscle pain, pork, fever, ELISA, Vietnam

https://drive.google.com/file/d/0BwAVbkCHjoJ8M1FvZDAtZ2xDYW8/view?usp=sharing